Inhibición de las encimas por el sustrato (con Cobra4)

Num.Producto: P4120460

Principle

The enzymatic hydrolysis of urea in aqueous solution liberates carbon dioxide and ammonia. The ions of these compounds increase the conductivity of the solution. Inhibition of the enzyme by the substrate occures at excessive substrate concentrations.

Benefits

  • Compact, easily transportable setup
  • For both demonstration and student experiments
  • Flexible experimenting thanks to wireless data transmission
  • Additional experiment in plant physiology and biochemistry can be performed with few modifications
  • Data acquisition with tablets possible
  • With detailed experiment guide

Tasks

  • Measurement of conductivity to determine the rate of hydrolysis of urea by the enzyme urease at various substrate concentrations

What you can learn about

  • Substrate inhibition
  • Enzymolysis of urea
  • Conductivity-time plot
  • Reaction velocity of enzymatic hydrolysis


Software included. Computer not provided.


Lista de material (extracto) Producto Cantidad
CurricuLAB-MeasureLAB, Software para mediciones y evaluaciones 14580-61 1
Cobra4 Wireless/USB-Link Adquisición de Datos con portátil, tableta o smartphone, incl. cable USB 12601-10 1
Balanza compacta, OHAUS TA 302, 300 g / 0,01 g 49241-93 1
Solución de ureasas en glicerina al 50%, 10 ml 31924-03 1
Agitador magnético con calefacción, acero inoxidable, digital, 280 °C, 100-1500 rpm FHO-RSM10HS 1
Cobra4 Sensor-Unit Conductivity+, Conductividad / Temperatur 12632-00 1
Electrodo conductividad/temperatura, Pt1000 13701-01 1
JERINGA MICROLITRICA,100 MICRO-L 02606-00 1
Soporte para mechero Bunsen 75 cm 37694-00 1
Varilla recoge imanes, resistencia química 35680-03 1
Matraz Erlenmeyer, cuello angosto, 100ml SB 19 36418-00 7
PERA PARA PIPETA 36592-00 1
Cargador USB para Cobra4 Mobile-Link 2 y Wireless/USB-Link 07932-99 1
Pinza en cruz 02043-00 1
Pinza universal 37715-00 1
AGUA DESTILADA, 5000ML 31246-81 1
UREA, 250 g 30086-25 1
PIPETA VOLUMETRICA, 50 ML 36581-00 1
PIPETA VOLUMETRICA, 20 ML 36579-00 1
Microespátula de acero inoxidable, l=150 milímetros 33393-00 1
Varilla para agitador magnético, cilíndrica, 30 mm 46299-02 1
Botella de lavado, plástica, 500 ml 33931-00 1
Vaso de precipitación, forma baja, BORO 3.3, 250ml 46054-00 1
V.D.PRECIP.,ALTO,BORO 3.3,100ml 46026-00 8
Tapón de goma, 17/22 mm, sin perforación 39255-00 7

A continuación la literatura para este producto

Título Producto Idioma
curricuLAB ExperimentierManager (online), Schullizenz 14575-62
curricuLAB measureLAB 14580-61
Basis-Set Cobra4 Biochemie und Pflanzenphysiologie für PC und Tablet 15620-88

regresar a la lista correspondiente

Product No: P4120460

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Contacto PHYWE

PHYWE Systeme GmbH & Co. KG
Robert-Bosch-Breite 10
D-37079 Göttingen

T. +49 (0) 551 604-0
F. +49 (0) 551 604-107
Línea directa de servicio técnico
+49 (0) 551 604-196
service@phywe.com

 

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